- What enzyme joins Okazaki fragments together?
- What do we mean when we say the two strands of DNA are antiparallel?
- How is RNA different from DNA?
- Why are new nucleotides added to 3 end?
- Which enzyme catalyzes the elongation of a DNA strand in the 5 → 3 direction?
- Is RNA built 5 to 3?
- Do you read DNA from 5 to 3?
- Why do Okazaki fragments form?
- What is the function of DNA polymerase III?
- Why is DNA not synthesized 3 5?
- What happens at the 5 end?
- Why does DNA polymerase 3 need a primer?
- What does 5 to 3 direction mean?
- Is the leading strand 5 to 3?
- What is the 3 end of DNA?
- What is the 5 and 3 end?
- Why does DNA synthesis only proceed in the 5 to 3 direction?
- Why are nucleotides added in the 5 to 3 direction?
- Why are nucleotides added in the 5 to 3 direction quizlet?
What enzyme joins Okazaki fragments together?
DNA ligaseThe strand with the Okazaki fragments is known as the lagging strand.
As synthesis proceeds, an enzyme removes the RNA primer, which is then replaced with DNA nucleotides, and the gaps between fragments are sealed by an enzyme called DNA ligase..
What do we mean when we say the two strands of DNA are antiparallel?
The term ‘antiparallel’ means that the strands run in opposite directions, parallel to one another. Each DNA molecule has two strands of nucleotides Each strand has a sugar-phosphate backbone, but the orientation of the sugar molecule is opposite in the two strands.
How is RNA different from DNA?
There are two differences that distinguish DNA from RNA: (a) RNA contains the sugar ribose, while DNA contains the slightly different sugar deoxyribose (a type of ribose that lacks one oxygen atom), and (b) RNA has the nucleobase uracil while DNA contains thymine.
Why are new nucleotides added to 3 end?
It keeps every cell division on the same page, so to speak. Because DNA synthesis can only occur in the 5′ to 3′ direction, a second DNA polymerase molecule is used to bind to the other template strand as the double helix opens.
Which enzyme catalyzes the elongation of a DNA strand in the 5 → 3 direction?
DNA polymerase IIIAnswer: C) DNA polymerase III catalyzes the elongation of a DNA strand in the 5′ → 3′ direction.
Is RNA built 5 to 3?
RNA growth is always in the 5′ → 3′ direction: in other words, nucleotides are always added at a 3′ growing tip, as shown in Figure 10-6b. Because of the antiparallel nature of the nucleotide pairing, the fact that RNA is synthesized 5′ → 3′ means that the template strand must be oriented 3′ → 5′.
Do you read DNA from 5 to 3?
5′ – 3′ direction refers to the orientation of nucleotides of a single strand of DNA or RNA. … DNA is always read in the 5′ to 3′ direction, and hence you would start reading from the free phosphate and finish at the free hydroxyl group.
Why do Okazaki fragments form?
Okazaki fragments form because the lagging strand that is being formed have to be formed in segments of 100–200 nucleotides. This is done DNA polymerase making small RNA primers along the lagging strand which are produced much more slowly than the process of DNA synthesis on the leading strand.
What is the function of DNA polymerase III?
The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.
Why is DNA not synthesized 3 5?
Normal DNA polymerases are 5′-to-3′ polymerases. DNA polymerases extend the 3′ tail of the DNA molecule but it synthesizes 5′-to-3′. 3′ to 5′ polymerases would never work because the energy required would be way too high. … DNA polymerases extend the 3′ tail of the DNA molecule but it synthesizes 5′-to-3′.
What happens at the 5 end?
What happens at the 5′ end of the primary transcript in RNA processing? it receives a 5′ cap, where a form of guanine modified to have 3 phosphates on it is added after the first 20-40 nucleotides. … They help ribosomes attach to the 5′ end of the mRNA once it reaches the cytoplasm.
Why does DNA polymerase 3 need a primer?
DNA polymerases add nucleotides to the 3′ end of a polynucleotide chain. … To initiate this reaction, DNA polymerases require a primer with a free 3′-hydroxyl group already base-paired to the template. They cannot start from scratch by adding nucleotides to a free single-stranded DNA template.
What does 5 to 3 direction mean?
DNA sequences are usually written in the 5′ to 3′ direction, meaning that the nucleotide at the 5′ end comes first and the nucleotide at the 3′ end comes last.
Is the leading strand 5 to 3?
One new strand, which runs 5′ to 3′ towards the replication fork, is the easy one. This strand is made continuously, because the DNA polymerase is moving in the same direction as the replication fork. This continuously synthesized strand is called the leading strand.
What is the 3 end of DNA?
3. Each DNA strand has two ends. The 5′ end of the DNA is the one with the terminal phosphate group on the 5′ carbon of the deoxyribose; the 3′ end is the one with a terminal hydroxyl (OH) group on the deoxyribose of the 3′ carbon of the deoxyribose.
What is the 5 and 3 end?
3′ end/5′ end: A nucleic acid strand is inherently directional, and the “5 prime end” has a free hydroxyl (or phosphate) on a 5′ carbon and the “3 prime end” has a free hydroxyl (or phosphate) on a 3′ carbon (carbon atoms in the sugar ring are numbered from 1′ to 5′).
Why does DNA synthesis only proceed in the 5 to 3 direction?
Why does DNA synthesis only proceed in the 5′ to 3′ direction? Because DNA polymerases can only add nucleotides to the 3′ end of a polynucleotide strand. … The final product of DNA replication is: two DNA molecules, each of which contains one new and one old DNA strand.
Why are nucleotides added in the 5 to 3 direction?
DNA replication goes in the 5′ to 3′ direction because DNA polymerase acts on the 3′-OH of the existing strand for adding free nucleotides.
Why are nucleotides added in the 5 to 3 direction quizlet?
why are nucleotides added in the 5′ to 3′ direction? The new complementary DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5 → 3 direction. … Many such segments are joined together to make up the lagging strand of newly synthesized DNA.